The "Law & Order: Special Victims Unit" star has brought on Courteney Cox, Amy Poehler and Ice-T for the PSA, made on behalf of the Joyful Heart Foundation's "No More" drive.
Actors Debra Messing and Tate Donovan also appear in the campaign.
The PSAs aim to raise awareness and also encourage bystanders to help victims, and hope their logo will become synonymous with raising awareness for the cause.
"This is an epidemic that is afflicting our society and I believe that it is an epidemic we can change. For the first time in history, the domestic violence and the sexual assault movements, the two communities, are coming together with this symbol," Hargitay said.
"It's to this movement what the pink ribbon was to breast cancer, what the red ribbon did for HIV/AIDS; it got people together, under one umbrella, talking about it.
"The shame and stigma belongs with the perpetrator. Let's not stand by; let's not accept it anymore. No more."