TV Guide reports the "Star Trek" star will also be joined by Brian Baumgartner from "The Office."
"At long, long last, I am working with someone older than me," Shatner, 82, joked in a statement.
"Hot in Cleveland" is an American sitcom on TV Land starring Valerie Bertinelli, Jane Leeves, Wendie Malick and Betty White.
Shatner's guest appearance will air live during the show's premiere of the second half of its fourth season on Wednesday June 19.
Celebrity Families of 2014 [PHOTOS]