American Crossroads, the conservative organization founded by Ed Gillespie and Karl Rove, put up $10,000 in digital advertising for the spot, which will run for two weeks, according to Roll Call.
The mock campaign ad satirically casts Judd as an Obama-loving Hollywood liberal who looks down on Kentuckians.
Judd's rep released a statement in response to the ad saying, "Ashley thanks Senator McConnell, Karl Rove, and their negative allies for all the attention as she considers her future political plans, although a decision hasn’t been made yet."
Judd told The Louisville Courier-Journal last month that she was "taking a close look" at a run for office.
"The people of Kentucky need a fighter," Judd said. "And certainly going back 10 generations, I've got some fighters from those hills in my family."