Farzana Parveen, 25, was stoned to death May 27 outside Lahore High Court. She was on her way to testify that she willingly married her husband, Muhammad Iqbal, willingly.
The marriage was against her family's wishes.
A Pakistani court indicted Parveen's father, two brothers, a cousin and a man who said he was previously married to her on murder and torture charges.
Police investigator Mian Zulfiqar said all five men pleaded not guilty to the charges.
The trial for the men is scheduled to begin Monday.
So-called honor killings are illegal in Pakistan. The Human Rights of Pakistan said 869 women were killed in 2013 in the country over familial honor.