The marbles, donated by Toosje Kupers, went on display Tuesday as part of an exhibit in Rotterdam, "The Second World War in 100 Objects," DutchNews.nl reported.
Kupers, 83, told Nos Television she had no idea the marbles would attract so much attention. She has donated them to the Anne Frank House organization in Amsterdam.
"To me, they were just a few marbles," she said.
Kupers lived next door to Anne Frank's family when Germany invaded the Netherlands in 1940. In 1942, when the Frank family went into hiding, Anne gave her friend the marbles and some other items.
Kupers has already donated a tea set and a book to the Anne Frank House.
The Franks were betrayed in 1944 and sent to concentration camps. Anne died in Bergen-Belsen in the spring of 1945.
Otto Frank, Anne's father, was the only survivor of her family. After the war, Miep Gies, a former employee who had helped hide the Franks, gave him Anne's diary, which was published as "Het Achterhuis" or "The Secret House" in the Netherlands and "Diary of a Young Girl" in English.