The accident occurred at about 6:30 a.m. as the bus was making its way to a baseball game, Japan Today reported.
The bus hit the car at an intersection, police said. The bus was knocked on its side.
The bus driver, the 18-year-old driver of the car, and 29 students were transported to a local hospital.
Police have not yet determined the cause of the accident.
2014: NFL Cheerleaders [PHOTOS]
N. Korea to bar foreign entry due to Ebola fear