In an interview first broadcast by Britain's ITV, Knox said Monday she hoped Kercher's family could "eventually take into consideration even the possibility of my innocence."
"I want them to understand that Meredith really was my friend, she was very kind to me and I had nothing to do with her murder," Knox said. "I am truly innocent and I truly believe that the only way that any of us are going to be able to heal from any of this is if we come together and acknowledge the pain that we have all gone through."
Knox and her boyfriend at the time, Raffaele Sollecito, were convicted of murder in Kercher's death, but that 2009 conviction was overturned on appeal. Italy's top court returned the case to the appeals court.
The second trial is scheduled to begin Sept. 30 in Florence.
Knox, who has always maintained her innocence in the 2007 death, said, "A lie detector test, I would be fine with that."
She said she was trying to rebuild her life following the "ultimate nightmare" of being wrongfully convicted and imprisoned in the death of Kercher, a Leeds University student, in November 2007.
Knox told ITV it was "so scary to have to go through this [a retrial] again."
She said she would not return to Italy for the new trial.