The protesters, some of whom were involved in the 1988 demonstrations, marched throughout the city to remember the uprising, during which more than 3,000 people were killed, Voice of American reported.
Demonstrators refused to stop along the route when police asked them if they had complied with a law requiring state approval for protests.
Meanwhile, British Foreign Office Minister Hugo Swire released a statement marking the anniversary.
"This anniversary is a chance to remember all those who have struggled for greater democracy in Burma, in particular the many who lost their lives in 1988 or spent years in prison because of their beliefs," Swire said. "The British government will continue to work together with the Burmese [Myanmar] government, opposition forces including Aung San Suu Kyi and the National League for Democracy, and all of Burma's ethnic groups to support the desires of the people of Burma for peace, and greater economic and political reform."