Bulls from the Valdefresno estate in Salamanca replaced the Cebada Gago animals, which veterinarians disqualified just days before Saturday's start of the San Fermin festival, EFE reported.
The Valdefresno bulls needed just 2 minutes, 28 seconds to complete the half-mile run from the Santo Domingo stockyard to the bullring.
The San Fermin festival, which ends Saturday, is known worldwide for its running of the bulls and revelry.
The festival, which began about 400 years ago, became popular after U.S. author Ernest Hemingway included it in his 1926 novel "The Sun Also Rises."
Four people were gored at the 2012 San Fermin festival.