Most were detained while attempting to hold a rally at City Hall in the Russian capital, RIA Novosti reported. Officials had denied a permit for an event there.
In Kiev, the capital of Ukraine, 50 activists were permitted to rally. Police kept a group of 400 anti-gay demonstrators away.
In Moscow, several people were arrested at the Duma, or parliament, building. Activists attempted to arrange consecutive pickets by single people there, which does not require a permit, but the demonstrators were arrested anyway.
One counter-protestor was arrested at the Duma for attacking two lesbian activists.
The Russian government has stepped up laws aimed at homosexuals recently. A bill creating the crime of "gay propaganda among minors" has been adopted in several regions, and a national version passed its first reading in the Duma this year.