The families of the defendants made the transfer request, al-Masry al-Youm reported, fearing the defendants would be targeted for more violence before the verdict announcement Saturday.
The Ismailia Appeals Court will only allow lawyers and media when the ruling is announced to prevent more violence, al-Masry al-Youm said.
The move comes after clashes in Port Said Sunday, and Thursday when protesters demonstrated for the fourth day demanding the prisoners be released.
The number of injuries in the clashes had reached 223, a media representative for the Health Ministry said.
Authorities said the defendants would stay in Ismailia until the ruling.