The Hollywood actor and the Real Madrid soccer player had been called to testify in the trial by the defense, Italy's ANSA news agency reported.
The judges said their testimony would have been "superfluous."
Berlusconi is accused of having sex with a Moroccan dancer, known as Ruby the Heart-stealer, when she was 17 -- a year under the legal age for prostitution in Italy.
Judges in the trial also said the verdict will not come until after next month's Italian general elections. Italy's elections take place Feb. 24-25, while the last hearing in Berlusconi's trial is set to take place March 11.
The court's announcement came after Berlusconi's defense lawyers again requested the trial be suspended until after the elections, arguing Berlusconi, who seeking to become prime minister again, will be too busy campaigning and there was a danger of rivals exploiting the trial.