Buses brought participants to the march from throughout Israel, The Jerusalem Post reported.
Association for Civil Rights in Israel President Sami Michael addressed the crowd at Rabin Square with a speech dedicated to women's rights.
"Billions are invested in national defense, but very little is in invested in defending women who on a daily basis face oppression, harassment, violence and murder," Michael said. "Discrimination against women means that society loses out on their enormous and blessed contribution."
The march in Tel Aviv came ahead of International Human Rights Day, which will take place Monday.
Dov Henin, a Knesset member from the left Hadash party, described the event as "a different type of politics, of values and cooperation, which is the answer to the great damage [done to] faith in democracy."