Members of the "I won't pay" movement raised the gates at toll posts for several hours across Greece, letting motorists roll on by for free, the Athens News Agency reported.
The organizers say they oppose toll hikes when the contractors have not built alternative access roads for local communities.
"We pay taxes on fuel and road taxes so that roads can be built," protest organizer Stratis Loupatatzis told the news agency. "We will not pre-pay road tolls as well."
He said the protesters are ready to go to court to get the toll road concessions contracts canceled.
Greeks take this issue seriously. Hundreds of thousands of drivers refused to pay the tolls during the Christmas period. And last month, Stylida Mayor Apostolos Gletsos, who also is a television actor, became a national hero to many when he drove a municipal bulldozer through the barriers of a toll booth to make his displeasure clear, The Guardian reported at the time.
"What I did was within the realm of my duties to defend the legal rights of citizens. I don't regret it and would do it again," said Gletsos who was arrested and charged.