Upon arrival, the princesses of York went out for a night out on the town on the city's iconic Beale Street.
Meanwhile, William and Harry joined friend Guy Pelly for a dinner at the Rendezvous restaurant to celebrate Pelly's upcoming nuptials to Lizzy Wilson.
Harry was "at the top of the stairs chatting with people who were drinking beers" at the restaurant a source told E! News.
"The upstairs was packed. Harry was talking to four or five guys. They were really happy and laughing. It seemed like they were having a fun party," the source added.
William and Harry are set to serve as ushers at Pelly and Wilson's wedding.