City Commissioner Joe Kilsheimer unseated Land in a decisive victory on Tuesday, garnering 54 percent of the vote to Land's 48 percent.
Land, who served as Apopka mayor for all but three years since 1949, spent more than twice as much money on his campaign than his opponent.
He told supporters that he was devastated by the loss.
"I love Apopka still, but I'll probably have a broken heart," he said.
Mayor-elect Kilsheimer said after his win that the city needed new leadership.
"For the first time in our city's history, both the old and new residents of Apopka are looking to have a say in how our city is run," he said.
[My News 13 Orlando, Orlando Sentinel]