The players had sued the city under federal civil rights law because they were forced to provide DNA samples to city police, but were never charged in an alleged rape.
In February, Duke University and 38 former members of its lacrosse team notified a federal judge they settled a players' lawsuit out of court, both sides announced.
The settlement left only one case against the school outstanding from the aftermath of a controversy after a stripper, Crystal Mangum, falsely claimed to have been raped at a team party in 2006.
The settlement ended all claims of the 38 players against the school and its police and health systems, the (Durham, N.C.) Herald Sun reported.
Though most dropped their suit, three players continued to pursue a civil rights case against Durham. But a federal appeals court narrowed the scope of their claims and the case was eventually dismissed.
The U.S. Supreme Court rejected review in a one-sentence order, only saying that Justice Samuel Alito did not participate in consideration of the case.
The order did not explain why Alito did not participate.