James Wise, director of the county Office of Emergency Management, said the collision occurred at about 1:30 p.m. EDT on U.S. 250 on Cheat Mountain, WCHS-TV, Charleston/Huntington, W.Va., reported.
Two passenger cars of the New Tygart Flyer -- operated by Durbin & Greenbrier Railroad -- tipped over, the report said.
Wise said all of the injured passengers were transported to Davis Memorial Hospital in Elkins, W.Va.
On its website, Durbin & Greenbrier Railroad says the New Tygart Flyer traverses "two separate mountain grades, an 'S' curve tunnel, passage into a 1,500 feet deep canyon with steep, densely forested slopes, and a high bridge over the rushing Shavers Fork of the Cheat River."
The rail line says the train "has gained a reputation as one of the most exciting mountain wilderness excursion rides" in the United States.
West Virginia Gov. Earl Ray Tomblin issued a statement saying his administration is working with all the responding agencies in response to the crash.
"Our thoughts and prayers are with all of those involved and the emergency responders working the tragic accident in Randolph County this afternoon," Tomblin said.