The crash happened Saturday as a van from Maranatha Seventh-day Adventist Church in Lauderhill drove to Tampa, The Miami Herald reported.
One of the van's tires blew near Fort Myers, causing the van to flip over. Nostra Damas, 20, died Saturday.
Claudine Aveske, 2, and Evelie Jean Francois, 39, died Sunday.
The van was carrying 16 people at the time of the crash, the South Florida Sun Sentinel reported.
The other 13 passengers were injured in the crash.
The Florida Highway Patrol said they would verify that everyone was wearing seat belts.
NBC reportedly holds celebs hostage to Jimmy Fallon's show
Aaron Carter is still in love with Hilary Duff