The group's earlier video, "Kony 2012," has been viewed at least 111 million times, making it one of the most-watched videos of all time, the Los Angeles Times reported.
The new video, "Move," was posted on YouTube late Sunday. Volunteers with Invisible Children hope to show the video hundreds of times at schools and community halls around the country to generate interest in a Nov. 17 rally in Washington to urge national leaders in the United States, Africa and Europe to increase efforts to capture Kony.
Kony and his followers are accused of allegedly capturing young children and using them as either child soldiers or sex slaves. He is believed to have fled Uganda in 2006 and to be hiding in central Africa.
The drive to capture Kony generated by the spring video was short-circuited when Jason Russell, the film's producer and the organization's founder, was arrested for running naked through the streets of San Diego, spouting gibberish.
Russell got medical treatment and withdrew into privacy for a while. His family has said he suffered a nervous breakdown brought on the intense publicity created by the "Kony 2012" video.