Country singer Lane Turner sang "I Built It" during the evening session of the first full day of the GOP confab at the Tampa Bay Times Forum.
The chorus includes the lines: "I bought it. A business I could call my own. I built it. With my own two working hands. No help from Uncle Sam."
As Turner sang, delegates waved placards proclaiming "We built it." Images of workers flashed on the screens on the stage.
Republicans seized on a comment Obama made in July.
"If you've got a business, you didn't build that," Obama said, referring to government-funded tools that entrepreneurs have at their disposal.
Republican speakers at the convention are using the "you didn't build that" comment to charge that Obama doesn't understand how a national economy grows and is anti-business.
The full quote is, as reprinted by The Washington Post: "If you were successful, somebody along the line gave you some help. There was a great teacher somewhere in your life. Somebody helped to create this unbelievable American system that we have that allowed you to thrive. Somebody invested in roads and bridges. If you've got a business, you didn't build that. Somebody else made that happen."