The billboard was installed on the Billy Graham Parkway Wednesday, The Charlotte Observer reported.
It was put up by San Diego-based Missiongathering Christian Church in response to 60 percent of North Carolina voters showing support for Amendment One, which defines marriage as between one man and one woman, when they went to the polls May 8.
The billboard reads: "Missiongathering Christian Church is sorry for the narrow-minded, judgmental, deceptive, manipulative actions of those who denied rights and equality to so many in the Name of God."
Amendment One was supported by the two Roman Catholic bishops of North Carolina and also got the support of some other churches across the state, with few churches opposing the amendment.
Missiongathering Church's mission statement says the church serves a diverse congregation and teaches a message of inclusiveness.