The news magazine's annual poll released this week shows the top-ranked school's tuition and fees for the 2010-11 school year are $38,426.
Riding Harvard's heels is Princeton University, lodged in the No. 2 spot and costing students (or parents) a cool $36,640 for tuition and fees.
Schools in the "National Universities" category, offer a broad range of undergraduate majors, master's and doctoral degrees and are committed to groundbreaking research, the magazine said.
Rounding out the top five universities are Yale and Columbia with the University of Pennsylvania and Stanford tied for fifth.
Other top schools include the California Institute of Technology and the Massachusetts Institute of Technology, tied for seventh; Dartmouth College in eighth; and Duke University and University of Chicago tied for ninth.
The first public school on the the National Universities list is University of California-Berkeley, occupying the 22nd slot.