The rights group, headquartered in New York City, said in a written statement that the incidents, which included 29 reports of physical violence and bomb threats, indicated a "sustained and troubling" level of anti-Semitism.
"The fact that Jews continue to be singled out for acts of hate on an average of three times per day in this country is a disturbing reality that we have to confront," said ADL National Director Abraham Foxman.
The incidents included the shooting of a security guard at the U.S. Holocaust Memorial Museum in Washington and a foiled plot by Muslim extremists to bomb New York-area synagogues.
Lesser-known incidents included a brick hurled at a Jewish man and aggressive picketing of community centers by members of a Kansas church.
The ADL said the number of incidents in 2009 was 10-percent lower than 2008; however the decline was attributed to a new reporting methodology rather than an actual decrease in incidents.