Russian Gen. Nikolai Makarov was quoted by state-run news agency RIA Novosti as saying there was "reliable evidence" to suggest Syrian rebels were using the Stinger missile, a man-portable air defense system.
"We need to look into" who delivered them, he added.
The Russian news service quotes a U.S. State Department spokeswoman as saying the government was working to "withdraw MANPADS from circulation."
Stingers deployed to local forces in Afghanistan were attributed to turning the tide against Soviet forces in the 1980s. RIA Novosti reports Internet videos show Syrian rebels using Soviet-era Strela missiles, a MANPAD, in Aleppo.
The claims follow reports from Human Rights Watch that claim Syrian forces have used Soviet-era cluster bombs during the civil war.
"Syria's air force is imposing a reign of terror on civilians in rebel-held areas across the country with cluster bombs and other explosive weapons dropped from aircraft," Steve Goose, arms director at Human Rights Watch, said in a statement.
Notable deaths of 2014 [PHOTOS]