Gadhafi in an audio message ruled out talks with rebels or members of the Transitional National Council.
"There will be no talks between me and them until Judgment Day," he was quoted by al-Jazeera as saying.
NATO members during a July 15 meeting in Istanbul recognized the TNC as a legitimate force in Libya. Washington said the conflict in Libya won't end with Gadhafi in power. White House spokesman Jay Carney, however, said "it is up to the Libyan people to decide what his future is beyond that."
French Foreign Minister Alain Juppe said Wednesday Gadhafi could stay in Libya provided he completely gave up power.
Meanwhile, rebel forces are apparently interrogating captured regime Gen. Abdul Nabi Zayed, which al-Jazeera says marks the highest valued Gadhafi loyalist detained by opposition forces since the conflict began in March.
The broadcaster's correspondent in Misurata said it marks a "significant catch" because fighters opposing Gadhafi are making "significant territorial gains."
The U.N. Security Council sanctioned international force in Libya in March. Gadhafi is wanted by the International Criminal Court for war crimes committed against the Libyan people since February.