Defense attorney Joel Brodsky alleged Judge Carla Alessio Policandriotes is biased against his 55-year-old client and should be replaced, the Chicago Sun-Times reported Saturday.
Peterson is a former Bolingbrook, Ill., cop accused of killing Kathleen Savio, his third wife.
Savio's body was found in 2004 and while her case was initially ruled an accidental drowning, Peterson now stands charged in her death.
The request to have Policandriotes removed marked the second time defense lawyers have attempted to have the judge replaced, the Sun-Times said.
Peterson's lawyers previously attempted unsuccessfully to have Policandriotes remove herself from the case, claiming she had links to the Peterson-Savio divorce case.
The newspaper said the substitution request means Will County Chief Judge Gerald Kinney will pick a new judge for Peterson's case, potentially in time for the murder suspect's June 17 hearing.
Peterson also is suspected, but not charged, in the October 2007 disappearance of his fourth wife, Stacy Peterson.