Mabel and Salvador Mangano, who own St. Rita's nursing home near Poydras, La., about 20 miles southeast of downtown New Orleans in St. Bernard Parish, entered their pleas in state district court in Chalmette before Judge Jerome Winsberg.
Winsberg did not set a trial date, but said Dec. 7 and Dec. 8 would be hearing dates for pre-trial motions, The (New Orleans) Times-Picayune reported.
Several relatives of St. Rita's residents who died in the hurricane gathered at the courthouse, some carrying signs with pictures of loved ones.
The Manganos remain free on bond.
Celebrity Families of 2014 [PHOTOS]