Winston completed 19-of-31 passes, including three scoring tosses to Kelvin Benjamin, who racked up 212 receiving yards for the unbeaten Seminoles (12-0).
Florida State will face upstart Duke in the ACC championship game before a potential berth in the national championship bowl.
Roberto Aguayo kicked three field goals for Florida State. The defense kept the Gators (4-8) off the scoreboard until the fourth quarter when Hunter Joyer caught a 5-yard TD pass from Skyler Mornhinweg to make it 27-7.
Mornhinweg completed 20-of-25 passes for 115 yards. Florida, which lost its last seven games of the season, converted only 1-of-11 third-down situations.