Gillislee's 37-yard touchdown run following a Seminoles turnover -- their fifth of the game -- put Florida (11-1) ahead 23-20 with 11:01 to go as the Gators reeled off 24 straight fourth-quarter points to rally for the victory.
Jeff Driskel returned from an ankle injury to compile 147 yards and a touchdown on 15-of-23 passing for Florida, which strengthened its shot for at-large Bowl Championship Series berth while besting the Seminoles for the first time in three years.
E.J. Manuel posting two rushing TDs and a passing score for Florida State (10-2), but completed just 18-of-33 attempts for 182 yards with three pickoffs and a lost fumble.
The Seminoles saw their five-game winning streak snapped in the run-up to next week's ACC championship game against Georgia Tech.