Scottie Wilbekin led the Gators (25-2, 14-0 SEC) with 18 points, and Michael Frazier scored 17, including five 3-pointers.
Wilbekin's jumper with 2:23 to play staked Florida to a 73-65 lead, which held up as the Gators put only two more points on the board. Ole Miss misfired on 4-of-5 tries from 3-point range in the final minute.
Marshall Henderson led Ole Miss (16-11, 7-7) with 22 points, but scored all of them in the first half. Jarvis Summers scored 20.
The score was tied 42-42 at halftime, but Florida outscored the Rebels 33-29 in the second.