The Gators won their 12th in a row, a streak that started with a victory over Kansas on Dec. 10.
After leading by only one at the half, Florida built as much as a 13-point cushion in the second period while remaining the only unbeaten team in Southeastern Conference play. Every other team in the league has at least two losses.
Prather finished with 15 points, Patric Young added 12 and Michael Frazier made three 3-pointers after missing his first six shots to wind up with 10 points. The Gators advanced to 18-2 for the season and to 7-0 in the SEC.
Florida sent Mississippi State to the foul line only six times and the Bulldogs converted just one of those chances.
Mississippi State (13-7, 3-4 SEC) was led by Colin Borchert with 15 points.