Ware was hurt in the first half of the top-seeded Cardinals' contest against No. 2 Duke with Louisville leading 21-20 when he hurtled toward the sideline while contesting a 3-point try.
The sophomore landed awkwardly and suffered what Louisville officials confirmed was a broken leg, causing visible distress among his Louisville teammates as well as among Duke's players and other onlookers.
A reporter for the Burlington (N.C.) Times-Union, who was seated nearby, wrote via Twitter Ware's bone was visibly protruding from his leg.
After a long delay, Ware was removed from the sidelines on a stretcher.
The gruesomeness of the injury was unprecedented in a major college basketball game, some observers said.
The team indicated Ware was taken to Methodist Hospital in Indianapolis in the company of his girlfriend and that his parents had been notified, CBS Sports reported.
Well-wishes for Ware came quickly via social media.
"Kevin Ware has been so important to Cards - very popular with coaches / teammates - wish him the best," wrote college basketball TV analyst Dick Vitale.