Barlow drove into paint past Jordan Hulls, spun to his right and put up the decisive shot, which rolled around the cylinder before dropping through for the Bulldogs (8-2).
The big basket came after Cody Zeller had tied the contest at 86-86 with 23 seconds in overtime for the Hoosiers (9-1), who fell from the ranks of the undefeated.
Indiana's Yogi Ferrell forced extra time when he connected on a 3-pointer for a 76-76 tie with 10 seconds left in regulation.
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