Tsonga entered the day fourth in the race for the final three invitations for the Tour Finals but dropped a 1-6, 7-6 (7-4), 7-6 (9-7) second-round decision to Kei Nishikori that eliminated him gaining a spot in the London Finals.
Tsonga lost his serve just once in the 2 1/2-hour match but that allowed Nishikori to get to the second-set tiebreaker. Tsonga fought off four break points -- he only saw six on the day -- in the third set but fell again in the tiebreaker.
Also in the second round, second-seeded Novak Djokovic extended his winning streak to 13 matches with a 7-6 (7-3), 6-3 win over No. 189-ranked Pierre-Hugues Herbert.
Herbert had a pair of set points but Djokovic fought those off and put together a string of points in the tiebreaker that seemed to break Herbert and led to a 5-0 Djokovic advantage in the second set.
It was Djokovic's first match since winning back-to-back tournaments in Beijing and Shanghai.
Ninth-seeded Richard Gasquet, just ahead of Tsonga in the Tour Finals standings, got by Fernando Verdasco 7-5, 6-7 (6-8), 6-3 and No. 14-seeded Jerzy Janowicz, last year's Paris Masters runner-up, beat Santiago Giraldo 7-6 (7-3), 6-3.
The first round was completed Tuesday with Marcel Granollers among the winners. He pulled off a 6-4, 6-4 win over Dmitry Tursunov, gaining the right to meet world No. 1-ranked Rafael Nadal in the second round Wednesday.
Grigor Dimitrov got past Michael Llodra 6-7 (5-7), 6-3, 6-3, losing his serve once in the process, and Kevin Anderson had just taken a 4-6, 7-6 (7-0), 2-1 lead when Mikhail Youzhny was unable to continue and retired.
Also Nicolas Mahut topped Alexandr Dolgopolov 7-6 (8-6), 6-1; Pablo Andujar, subbing for an injured Gael Monfils, got by Vasek Pospisil 6-4, 2-6, 6-4; Ivan Dodig stopped Edouard Roger-Vasselin 7-6 (8-6), 6-4; and Michal Przysiezny beat Jarkko Nieminen 6-3, 7-6 (8-6).