Wilson finished with 92 yards, giving him 402 for the season -- breaking Seattle's old QB rushing mark of 343 yards set by Rick Mirer in 1993.
He also completed 14-of-22 passes for 205 yards and a touchdown as surging Seattle (9-5) kept pace with first-place San Francisco (10-3-1) in the NFC West.
The 49ers defeated New England Sunday night.
Marshawn Lynch led the Seahawks' rushing corps, amassing 113 yards with a touchdown on 10 carries.
The Bills (5-9) were eliminated from post-season contention for a 13th straight year as Ryan Fitzpatrick was picked off twice.
C.J. Spiller contributed 103 yards with a touchdown on 17 carries for Buffalo.