The Williams sisters, who came into the tournament unseeded, bested No. 6-seeded Hlavackova and Hradecka 7-5, 6-4 at the All England Club for the doubles title.
The victory came only hours after 30-year-old Serena had collected her fifth Wimbledon singles title Saturday, prevailing over Agnieszka Radwanska in three sets.
The siblings earned their 13th Grand Slam doubles title, and their first major doubles title since the 2010 Australian and French Open titles.
2014: The Year in Music [PHOTOS]