With the Rays trailing 2-1 entering the frame, Desmond Jennings drew a leadoff walk from Chien-Ming Wang (2-3) and Pena followed by smashing an 0-1 pitch deep over the center field wall.
Later in the inning Ben Zobrist and Jose Lobaton were aboard with two outs and Johnson belted a Wang offering high off the left-field wall for a two-run triple.
David Price (9-4) earned the victory despite allowing four runs (three earned) on six hits in a seven-inning start. The left-hander walked one and struck out four as the Rays claimed the opener of a three-game series.
Wang ended up yielding five runs on seven hits, lasting only 3 1/3 innings.