Rondo made contact with official Marc Davis Sunday near the end of the Celtics' 83-74 playoff loss to the Atlanta Hawks and was ejected from the contest.
He was arguing a foul Davis had called on teammate Brandon Bass.
Despite Rondo's pleading the contact was incidental and caused by his momentum, the league ordered him to sit out Tuesday's Eastern Conference quarterfinal Game 2 in Atlanta.
"Obviously, from a competitive standpoint we are disappointed with the league's decision to suspend Rondo," Celtics Coach Doc Rivers said. "He plays a valuable part in our team's success. We accept the punishment and will use it as a learning tool for our players."
The Hawks won Sunday's Game 1 83-74.