Chamberlain was injured Thursday while playing with his son, Yankees General Manager Brian Cashman said. He had surgery Thursday night.
Chamberlain was rehabilitating after undergoing so-called Tommy John surgery in his right -- throwing -- elbow last summer. He was looking to return to the team this June.
Chamberlain, 26, pitched in 27 games last season, going 2-0 with a 2.83 earned run average. In five seasons with the Yankees, Chamberlain is 20-13 with a 3.70 ERA.