Gronkowski, who set an NFL record for touchdown catches by a tight end this season with 17, suffered a sprained ankle during New England's AFC title win over Baltimore and has seen limited practice time since.
His 90 receptions this season were second on the team behind Wes Welker's 122.
Gronkowski took part in New England's practice session Friday, the second straight day he had done so. Coach Bill Belichick said the tight end showed no ill effects from his work on Thursday.
Others listed as questionable for Sunday's game with the New York Giants were safety Patrick Chung (knee), guard Logan Mankins (knee), linebacker Rob Ninkovich (hip), linebacker Brandon Spikes (knee) and Welker (knee).
Every member of the Giants was listed as at least probable.
"So far this week, we've accomplished what we needed to accomplish," New York Coach Tom Coughlin said in his final news conference prior to Sunday's game. "The players have practiced hard and spirited. I think we'll be well-prepared for the game."