The announcement came 17 days after Crosby returned to the ice following an almost year-long absence brought on by a concussion.
"Sidney took a hard hit during our game against Boston Monday night and wasn't feeling 100 percent," Penguins General Manager Ray Shero said Wednesday.
Shero said tests for concussion-related symptoms were negative.
"However, we all think it's best that he sits out the next two games as a precaution," Shero said.
Crosby scored twice in his first game back, but has not had a goal since. He has picked up 10 assists in eight games.
The Penguins will play their next two games on the road against Philadelphia on Thursday and the New York Islanders on Saturday.