Before resting, prosecutors read statements Bonds made in 2003 to a grand jury investigating the Bay Area Laboratory Co-Operative Laboratory (BALCO), which had been supplying steroids to athletes, MLB.com reported.
Bonds, baseball's all-time, home run king, is accused of lying to that panel when he denied knowingly using performance-enhancing drugs.
Prosecutors Tuesday called as witnesses three employees of the UCLA Olympic Analytical Laboratory, who testified about how urine samples taken from Bonds in 2003 were handled.
The samples tested positive for the steroid THG and other banned substances.
Judge Susan Illston denied a government motion to allow a recently discovered tape recording made by previous prosecution witness Steve Hoskins, a boyhood friend and former business partner of Bonds, MLB.com reported.