Latos, who has a strained right shoulder, is one of four Padres placed on the DL.
Latos was 14-10 with a 2.92 earned run average in 31 appearances in 2010.
Utility player Mike Baxter and outfielder Eric Patterson went on the DL earlier.
San Diego, which went 90-72 last year, will take 12 pitchers, six infielders, five outfielders and two catchers with them for its season-opener Thursday in St. Louis.
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