Latos, who has a strained right shoulder, is one of four Padres placed on the DL.
Latos was 14-10 with a 2.92 earned run average in 31 appearances in 2010.
Utility player Mike Baxter and outfielder Eric Patterson went on the DL earlier.
San Diego, which went 90-72 last year, will take 12 pitchers, six infielders, five outfielders and two catchers with them for its season-opener Thursday in St. Louis.
Boston schools pull out free condoms over wrapping complaints
NBC reportedly holds celebs hostage to Jimmy Fallon's show