Miller put in a career-high 24 points, and Knight added 16 and six assists for the Wildcats (20-8, 8-6 SEC), who shook off an overtime loss to Arkansas Wednesday and posted their 33rd straight home win.
Doron Lamb added 14 points for Kentucky, which will conclude the regular season with games against Vanderbilt and Tennessee.
The Gators (22-6, 11-3), meanwhile, saw their six-game winning streak snapped despite 21 points from Kenny Boynton, and 15 points, eight rebounds and seven assists from Chandler Parsons.
Florida, which wraps up the season with contests against Alabama and Vanderbilt, can clinch the SEC East title outright with one more win.
Swim Week Miami Beach 2014 [PHOTOS]