John Wall chipped in with 11 points and eight assists in the win, which enabled the Wildcats (29-2, 14-2 SEC) to post a perfect 18-0 record at home this season.
Kentucky had already won the regular season SEC title outright and enters the conference tourney on a two-game winning streak. The Wildcats will likely be a No.1 seed in this month's NCAA Tournament.
Florida (20-11, 9-7) narrowed Kentucky's advantage to 50-46 and were within one possession several times, including with 5:23 to go, but then the Wildcats tightened up on defense, holding the Gators scoreless for more than three minutes.
Alex Tyus scored 12 points to lead Florida, while Kenny Boynton and Erik Murphy each chipped in with 11 in the loss.