Fans poured out the stands and onto to basketball court to celebrate the Gamecocks' victory that marked the first loss of the season for Kentucky, as the game ended Tuesday.
SEC rules set the fine at $25,000 as a second offense since the policy was put in place in December 2004. South Carolina was fined $5,000 when the Gamecocks beat Kentucky in 2005.
Any third violation would be a $50,000 fine.
"This policy is designed to create a safe environment for everyone who participates and attends our athletic contests," SEC Commissioner Mike Slive said in a statement. "The security and protection of our student-athletes, coaches, officials and fans is our primary concern."
SEC leading scorer Devan Downey had 30 points and the Gamecocks held Kentucky off down the stretch. The Wildcats had taken over the No. 1 position in the USA Today/ESPN Coaches' poll Monday.