The nine-year veteran missed last week's 31-26 loss to Baltimore after suffering the injury in San Diego's season-opening 24-20 win over Oakland.
It marks only the third time in his career that Tomlinson missed a game due to injury.
He wore a wear a protective boot before the Baltimore loss.
"I can get healthy," the 2006 NFL MVP told the San Diego Union-Tribune earlier this week.
He missed San Diego's AFC playoff loss to the Pittsburgh Steelers in January with a groin injury.
The bulk of the running attack likely will be shared by Michael Bennett and the diminutive Darren Sproles when the Chargers are home to the Miami Dolphins Sunday.
2014: The Year in Music [PHOTOS]