The game involved the last two National Champions for the first time since Notre Dame (1988 champs) defeated Miami-Florida (1989 champs) in 1990.
The Gators scored 21 straight second-half points to pull away for the victory. Tebow added a rushing touchdown for Florida (5-1, 3-1 SEC).
Freshman Jeffrey Demps rushed for 129 yards and a TD on just 10 carries for the Gators. Brandon Spikes led the Florida defense with five tackles and two interceptions, returning one 52 yards for a touchdown.
LSU's Jarrett Lee hit 23-of-38 passes for 209 yards with two touchdowns and a pair of interceptions. LSU (4-1, 2-1) had a six-game winning streak, dating back to last season, snapped.
LSU's Charles Scott was held under 100 yards for the first time in five games, getting just 35 yards on 12 carries.