Creamer was at 17-under 125 at the halfway mark. She had fired a 60 in the opening round, just missing the LPGA Tour's 18-hole record of 59 set by Annika Sorenstam.
Eun-Hee Ji carded a 66 to hold second place at 11-under 131. Rachel Hetherington (67) and H.J. Choi (68) were tied for third at 7-under 135.
"It was probably one of the hardest rounds that I've had," Creamer said. "You come out and shoot 60, and anything above 60 feels like you are not playing that great. I made some putts. At the same time I missed a lot of shots out there."